Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.068746 |
Chromosome: | chromosome 2 |
Location: | 2167061 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g089350 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR_intron | |
Cre02.g089400 | HRP2 | (1 of 12) 2.7.11.18 - [Myosin light-chain] kinase / Smooth-muscle-myosin-light-chain kinase; Hydroxyproline-rich glycoprotein | 3'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGCACGCCGGGAGCTTGGCTCGTACGGCACCTGGCCCGGCGCACGT |
Internal bar code: | TTGAGTTAGAGGGGAATTCCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2129 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 25 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCATGCACACACTGGATCC |
Suggested primer 2: | GACGACGACGACGATGATGA |