| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068750 |
| Chromosome: | chromosome 7 |
| Location: | 4187656 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342150 | GluTR,HEM1,HEMA,GTR,GLTR1 | (1 of 1) 1.2.1.70 - Glutamyl-tRNA reductase; Glutamyl-tRNA reductase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCAGCAGGTATGTACGCGGGGGCTCAGGGCGCCGGCCAGGGCACCAA |
| Internal bar code: | GCGAAGCGTTTGGAGCGTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4971 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACGTGCTTGGACTGTG |
| Suggested primer 2: | CCTCGCGCCATATACCAGTT |