| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068755 |
| Chromosome: | chromosome 14 |
| Location: | 3306293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629300 | (1 of 3) K11855 - ubiquitin carboxyl-terminal hydrolase 36/42 [EC:3.4.19.12] (USP36_42) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATGGCAACAATCCGTCTGACAGGGCGCAGGGACAGTGATGATACGGT |
| Internal bar code: | GCTGACTGACGCGCGACCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2378 |
| LEAP-Seq percent confirming: | 88.1356 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGGTCACTGAGTGTGG |
| Suggested primer 2: | GTACGATGGCTGGGATCAGG |