Insertion junction: CLIP2.068758_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.068758
Chromosome:chromosome 2
Location:2533756
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g092350 CYP51G1,CYP6 (1 of 1) 1.14.13.70 - Sterol 14-alpha-demethylase / Obtusufoliol 14-demethylase; Cytochrome P450, CYP51 superfamily 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTCATCGCGGCGCGTTGTGACATCCAATATCATAAGCTGATTTCACG
Internal bar code:CTTTTCGCATGAATTGGACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGATAGGACGAGGCTGTC
Suggested primer 2:TCCGTCAACTGAGGTCAAGC