| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.068770 |
| Chromosome: | chromosome 13 |
| Location: | 5171973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607300 | FAP352,MAPK5 | (1 of 1) K08293 - mitogen-activated protein kinase [EC:2.7.11.24] (E2.7.11.24); Flagellar Associated Protein 352 | 3'UTR |
| Cre13.g607350 | CAH7 | (1 of 2) PTHR11002:SF13 - BETA CARBONIC ANHYDRASE 1; Carbonic anhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGCCGTTTACTAGCTGCTGCTGTCCTACGCATCGTAGATATGCTCCG |
| Internal bar code: | TTTGCGTTCTTTTTCGCGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTTGCGCCTAGATGGTT |
| Suggested primer 2: | TGAGGATGAGAGGGAGGTGG |