Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.068791 |
Chromosome: | chromosome 3 |
Location: | 8625942 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g210177 | (1 of 3) PF13405//PF13499 - EF-hand domain (EF-hand_6) // EF-hand domain pair (EF-hand_7) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGATTATGCACTGATTGATTGATGTTACAAGTTCATGCTCGTAGATT |
Internal bar code: | TCCGTCTGGGGGTAAGGCGTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4079 |
LEAP-Seq percent confirming: | 76.4706 |
LEAP-Seq n confirming: | 26 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACAGTGCATAATCGGTGCG |
Suggested primer 2: | AACTCCGTGAGCGTCATTGT |