Insertion junction: CLIP2.068791_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.068791
Chromosome:chromosome 3
Location:8625942
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g210177 (1 of 3) PF13405//PF13499 - EF-hand domain (EF-hand_6) // EF-hand domain pair (EF-hand_7) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGGATTATGCACTGATTGATTGATGTTACAAGTTCATGCTCGTAGATT
Internal bar code:TCCGTCTGGGGGTAAGGCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:4079
LEAP-Seq percent confirming:76.4706
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGTGCATAATCGGTGCG
Suggested primer 2:AACTCCGTGAGCGTCATTGT