Insertion junction: CLIP2.068804_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.068804
Chromosome:chromosome 1
Location:4457373
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g030450 (1 of 1) IPR006615//IPR028881//IPR028889//IPR029071 - Peptidase C19, ubiquitin-specific peptidase, DUSP domain // PAN2 domain // Ubiquitin specific protease domain // Ubiquitin-related domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTGCCAGTTCGCTTCCTCCACAACAACAGTCCGCAACTACCTGAGCT
Internal bar code:TTCACAACGCTCTCGGTTCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3182
LEAP-Seq percent confirming:76.4706
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCTTGCATGACATGGTGC
Suggested primer 2:TGAACACGCGCCATAGCTTA