Insertion junction: CLIP2.068805_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.068805
Chromosome:plastome
Location:1978
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802263 ChreCp001,2716989,petA (1 of 1) K02634 - apocytochrome f (petA); apocytochrome f precursor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGGATATACTTGACCACGACCACGATTACCACCAAAATAAATAGGATA
Internal bar code:CGCGCGGTTCGATGAGGGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:57
LEAP-Seq percent confirming:4.54545
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCACGTGAGGCTAATGG
Suggested primer 2:GAAGGAGGCAGTGGAGGAAC