| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068817 |
| Chromosome: | contig 35 |
| Location: | 26050 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre35.g802212 | (1 of 2) PTHR18945:SF559 - PROTEIN LGC-12 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACTTTACACGGGACCTGCTTTGCCCGGCCCGACCTGCTTTGCCAAGCC |
| Internal bar code: | CATGAGGTCCACCGTGAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4041 |
| LEAP-Seq percent confirming: | 1.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGGGCTCCATTCGCTAC |
| Suggested primer 2: | CGTGGGTCAATTCAGCTCCT |