Insertion junction: CLIP2.068821_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.068821
Chromosome:chromosome 3
Location:8049928
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCGGGAGGGAAACTGCCCAAGACCGCGGGACGCACAGCGGCAGCCTT
Internal bar code:ATGAAGTTAGGTCTAGGGTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2619
LEAP-Seq percent confirming:98.6487
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTGTGGTGTGCAGTCTG
Suggested primer 2:GAGTGTGGAAGGTGCGTGTA