| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.068828 |
| Chromosome: | chromosome 5 |
| Location: | 2282007 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238100 | PTK25 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCGCTCGCCTCTGCGTGTACGCCCCCTCCCCCGCAACCTTGCCACC |
| Internal bar code: | GTGTCAAGCAGTGACTAATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGATGGAGACGTGGATCG |
| Suggested primer 2: | CTCAGTCCCACTTCCCACAC |