| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.068833 |
| Chromosome: | chromosome 11 |
| Location: | 2198457 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467725 | 5'UTR | ||
| Cre11.g467726 | (1 of 1) K06316 - oligosaccharide translocation protein RFT1 (RFT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGTGATAAACACCTTTTGGTTTGCCTCGCGACGAGTCTCTGTGGCG |
| Internal bar code: | CACGACGTAACGTTACGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATCAGCATAGGGGCCTG |
| Suggested primer 2: | ACGTTAGCGCAGAGTTGTCA |