Insertion junction: CLIP2.068839_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.068839
Chromosome:chromosome 6
Location:210889
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g250450 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7); Sodium:bile acid symporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTTGAGGAGCGGGAAAAATGGTGGAGGTCGCGGGCAACCCGGTGAAG
Internal bar code:ACGGGAGTTATTATTTCTTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5219
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCAGAATCATGGTCACCA
Suggested primer 2:TGCGTTAATCCTTGGGGCAT