| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068846 |
| Chromosome: | chromosome 16 |
| Location: | 2439101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660050 | CMT1B | (1 of 2) PTHR12608//PTHR12608:SF7 - TRANSMEMBRANE PROTEIN HTP-1 RELATED // GDT1-LIKE PROTEIN 2, CHLOROPLASTIC; Mn and/or Ca transport | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCAACAGTTGTGGATGTGATGATTGCGGGCGGTTGCGGAATGCTG |
| Internal bar code: | TTGCTTGGGATTTGAGTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1389 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTGGGGACTCGGGCATA |
| Suggested primer 2: | AAGTATGCACCTTCCCAGCC |