Insertion junction: CLIP2.068855_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.068855
Chromosome:plastome
Location:186256
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802330 orf2971,ftsH,ChreCp065,2716962 ATP-dependent zinc metalloprotease; (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGTACACTCATGGTGGCAAGTTCGATAAACTTTAGTTACCCGAAGGGG
Internal bar code:CGGATAACAATTCCGTTACGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:93
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTTTCTTGGTCACGGCC
Suggested primer 2:AAGCGTCGTCGTTGGTTACT