Insertion junction: CLIP2.068860_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.068860
Chromosome:plastome
Location:16361
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802269 ChreCp007,clpP,2717015 (1 of 9) 3.4.21.92 - Endopeptidase Clp / Protease Ti; ATP-dependent Clp protease proteolytic subunit 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACTAACTATTACTTTCTTGCGTTTGTGTACTATCACCTACTAAACTAG
Internal bar code:CGGCACTCCCAGCCTCCAAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:468
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTAGCTGCATCTGCTG
Suggested primer 2:CGAAGCCACGCCAATTTTCA