| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068865 |
| Chromosome: | chromosome 10 |
| Location: | 4431617 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450550 | PAO3,PAO1 | Pheophorbide a oxygenase; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCCTGGCCTGTCGCTTCAAGCTGGGTGCAGCTGCGAGGCGGCCGCG |
| Internal bar code: | AGTAGATACTGCTCCAGGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGCTATGCTCACAGCTA |
| Suggested primer 2: | ACACGCCCTCATAGAACAGC |