| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068866 |
| Chromosome: | chromosome 7 |
| Location: | 3769839 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338350 | CPLD50 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR10072//PTHR10072:SF43 - IRON-SULFUR CLUSTER ASSEMBLY PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
| Cre07.g338400 | (1 of 1) K14845 - RAT1-interacting protein (RAI1, DOM3Z) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTTTTCATATTTGCCGGTGCACTTGCAAGTAAGCCAGCACCACAGCA |
| Internal bar code: | CGGCCCGGCGTGGCACCTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2160 |
| LEAP-Seq percent confirming: | 92.9825 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCTCTACAGGGTTGG |
| Suggested primer 2: | TGCAAACCCCGTATGATGCT |