Insertion junction: CLIP2.068902_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.068902
Chromosome:chromosome 17
Location:6832357
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g747197 (1 of 1) K05758 - actin related protein 2/3 complex, subunit 2 (ARPC2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGACCACAACCAACAGCACTGCCCCTACACATTCATAACTCCACACACAC
Internal bar code:CGCGATATGTATACGGACAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCACTGTCCCATCGCAC
Suggested primer 2:AGCTTGAGAACGGGGAAGTG