Insertion junction: CLIP2.068905_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.068905
Chromosome:chromosome 6
Location:5052200
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g282150 CNX1G,CNX1C (1 of 2) 2.7.7.75 - Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCACACCGCGCTCCTCTCCACGCCGCCACACTGCCCTGTGGTGCCGGC
Internal bar code:AATTGTGTTTTTTGGAGGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:192
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AACCACCTGCTACTCGTGTG
Suggested primer 2:CGTAGGGTTCTGCACCTCAG