| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068911 |
| Chromosome: | chromosome 13 |
| Location: | 2967788 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801540 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAACATCCGCATCCATAGCAGGCCAGTCTATCAGGATACTTGTCGGC |
| Internal bar code: | TGTCCAGAAATTATCGCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1647 |
| LEAP-Seq percent confirming: | 14.4928 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCGTGGGCGTTTACCAT |
| Suggested primer 2: | TGGTTTTGGGACTGCACTGT |