| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068914 |
| Chromosome: | chromosome 5 |
| Location: | 186537 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242700 | SUB9 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCCGCACTCAGCTAAACGCGACGTAATCAATTCAGGTGGGCGTCCGC |
| Internal bar code: | GATGAAGGGGATCCGCTAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3224 |
| LEAP-Seq percent confirming: | 25.3731 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAGAGCTTGTGCATAGC |
| Suggested primer 2: | GGTGGTGGAGGACAAGGAAG |