| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068921 |
| Chromosome: | chromosome 3 |
| Location: | 534418 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145147 | TRP8 | Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | 5'UTR |
| Cre03.g800287 | (1 of 1) PF00665//PF13976 - Integrase core domain (rve) // GAG-pre-integrase domain (gag_pre-integrs) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCCCCGCGTGAGTGGCGGGTTTGCCCTTTATTTGTTAAGAGTCTAA |
| Internal bar code: | GACTGCAAAGGCGGATAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3402 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTCGCCTTGTTCCACT |
| Suggested primer 2: | TTTGCACACTGTCAACCCCT |