| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068922 |
| Chromosome: | chromosome 1 |
| Location: | 657135 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003700 | (1 of 2) PF10263 - SprT-like family (SprT-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGGTACGGACGGCACGGTGCGTGCGCGCGGCGGTGGCGGGCTGGGC |
| Internal bar code: | GCAGGATGTGGGCGATTCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAGAGGAGGACCCGAT |
| Suggested primer 2: | GAGAACAAGGGATGGGTGGG |