Insertion junction: CLIP2.068927_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.068927
Chromosome:chromosome 12
Location:9650474
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g558353 (1 of 11) PF00999 - Sodium/hydrogen exchanger family (Na_H_Exchanger) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCAACAGCGAGTCTACTCCTGCCACGCCAGCGGCGGGAGGGGCAGGGG
Internal bar code:TCTACCTCTGCCCAGGGGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:701
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTGCTTCAACATGCTTC
Suggested primer 2:CATGTCACTTGTGGCGTGTG