| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068934 |
| Chromosome: | chromosome 13 |
| Location: | 2679949 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581450 | RPN7 | (1 of 1) K03037 - 26S proteasome regulatory subunit N7 (PSMD6, RPN7); 26S proteasome regulatory subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGAGGCGCTAGGCCAGGGCACCCCTGCCCTAGGCACTCAGTTCCCC |
| Internal bar code: | TTTGTCCAAGACGGATGTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2636 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGGGATAAGTAGCATGGT |
| Suggested primer 2: | GTACTGGTAGAGAGGGGCCA |