| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068937 |
| Chromosome: | chromosome 1 |
| Location: | 4172059 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027900 | (1 of 1) K17338 - receptor expression-enhancing protein 1/2/3/4 (REEP1_2_3_4); similar to HAV22 | 3'UTR | |
| Cre01.g027950 | IFT74,CMG1,IFT71,IFT72 | (1 of 1) PTHR31432:SF0 - INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG; Intraflagellar transport protein 74/72 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTTTGTTGTAAAAGAGCCCACACAACAAGTACTCATTCCCGCATCGGC |
| Internal bar code: | GGGGAAACTCGGCGCATAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACGTTGTCCAGTCATCG |
| Suggested primer 2: | CAAGCTGTTGTTCGTGGTGG |