| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.068939 |
| Chromosome: | chromosome 5 |
| Location: | 1512140 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234657 | (1 of 1) 2.7.7.87 - L-threonylcarbamoyladenylate synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAATCGTGCTGGAATAGCTCGTTTATAGCGGCCTTACTCCAGTGAACA |
| Internal bar code: | TATTGACAGTTAGTACGCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACACGGCAGAGAGTTGA |
| Suggested primer 2: | CGCCGTAAACAGTTGACGAC |