| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068969 |
| Chromosome: | chromosome 6 |
| Location: | 1562410 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261050 | (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGCGGACACACTTGTACGCACACCTCCGCTTACTCTTTCTCAACCC |
| Internal bar code: | GACGGGGTGTGAGAGTCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGTGTACCTCATTGCCT |
| Suggested primer 2: | TAGGGTATGTGTGGAGGGGG |