| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068985 |
| Chromosome: | chromosome 3 |
| Location: | 6407830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800362 | (1 of 2) PTHR10925 - N-ACETYLTRANSFERASE 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTGAATGATTGTGGATGCTGCAAATCGTTACTTGGCGGTGTCGTCG |
| Internal bar code: | TGTTACTTAGATCTGTTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2119 |
| LEAP-Seq percent confirming: | 80.8824 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAAGGACAAGGGCGACA |
| Suggested primer 2: | CCCGTTCCTGACAACTTCGA |