| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069001 |
| Chromosome: | chromosome 4 |
| Location: | 3023021 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225100 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTACCTGCTGCCGCCGCCGCCACCGCCGCCAGCAGCAGCAGCAGCAG |
| Internal bar code: | TTTTCTTAAGGTTAGGACCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2379 |
| LEAP-Seq percent confirming: | 21.4286 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATGCGCAGTAAAGGGA |
| Suggested primer 2: | AACAGTACCACAGGCGTCAG |