Insertion junction: CLIP2.069013_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.069013
Chromosome:chromosome 6
Location:7315862
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGACTCGCGCGCATCGCTTTATTAGCAGCTGCTAAGCACTGCAAGCA
Internal bar code:GGAAAGATGTAAGAGTTTGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3502
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:94

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCAAACCAACATCAGCAA
Suggested primer 2:AAGGGCTGGGATCCATTGTG