| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069014 |
| Chromosome: | chromosome 7 |
| Location: | 2056820 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800789 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCCATCCTTTTTGTCATAGCCATGCTCGAGCCGCAGCGCAAGCTTA |
| Internal bar code: | GAAGTGGGGCCAATGTTAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 966 |
| LEAP-Seq percent confirming: | 26.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGCAACAGCAGACCAGC |
| Suggested primer 2: | CAGACGCCTGTGGTGGTATT |