Insertion junction: CLIP2.069024_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.069024
Chromosome:chromosome 3
Location:6300977
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192050 FTR1 (1 of 1) K07243 - high-affinity iron transporter (FTR, efeU); Iron permease 1, membrane protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGAACATGTGTAACGTGACGAGCGGCGCACTGTCCAGTTGCCAAAAA
Internal bar code:TGGTTTCGTTTTAGACTGTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAACCTGCGTGGCATATG
Suggested primer 2:CTAGTTCGAGCGCAATGTGC