| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069042 |
| Chromosome: | chromosome 10 |
| Location: | 2501046 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435950 | OPR47 | OctotricoPeptide Repeat protein 47 | 5'UTR |
| Cre10.g436000 | POC2 | Proteome of centriole protein 2; (1 of 2) PTHR10877//PTHR10877:SF135 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTCTCAGTCGCCAACTGCGTGTCATAGTGATTGTACAGGTCGTCCAG |
| Internal bar code: | ATCAAGTGTGCGCAGCACCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGTGTTCAACTGCCAC |
| Suggested primer 2: | CGTGTCGCCATACGGTATGA |