| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069048 |
| Chromosome: | chromosome 2 |
| Location: | 5547707 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115050 | RSEP3,RSE3 | (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease | 3'UTR |
| Cre02.g115100 | (1 of 1) IPR000945//IPR001810//IPR020683 - Dopamine beta-hydroxylase-related // F-box domain // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGGTGACCCGGCGAGCATATTTAAAAGTACATGAAGCAAATACACG |
| Internal bar code: | TCGGCCTGTAGTAAACGTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3424 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACTTATTTGCGGCGTGG |
| Suggested primer 2: | AAGAGTTGTGAGGCACGGAG |