| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069049 |
| Chromosome: | chromosome 2 |
| Location: | 2449380 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091550 | WHI,EZY18,PBF2 | (1 of 1) PTHR31745:SF1 - SINGLE-STRANDED DNA-BINDING PROTEIN WHY2, MITOCHONDRIAL; Whirly domain protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGTCACGTCATGTCCCTTTCCACGCCCACTGCAGGCGCCCGTCCC |
| Internal bar code: | TTCGAGTGAAACAGATGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 5.55556 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGCTTAGGGGCAGGAGT |
| Suggested primer 2: | TGGCATGAGGGCTGCATTTA |