Insertion junction: CLIP2.069057_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.069057
Chromosome:chromosome 12
Location:5616793
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g531350 CGL62 putative cell cycle associated protein; (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCCCGTGGGCGGCAGGTCCATCTGCAGGTTGCGTATATCACATGTGC
Internal bar code:AACTCGGTGTCATACCCGCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3030
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:AACATACCGCCTTGCTTCCA
Suggested primer 2:GGTGACACAACCGACCTCAT