| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069058 |
| Chromosome: | chromosome 5 |
| Location: | 1515430 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234658 | (1 of 1) PTHR13480:SF0 - E3 UBIQUITIN-PROTEIN LIGASE HAKAI | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCCTCGACACTTTCAGCGCGCGCACACACACGGTAGCACACGGGCTG |
| Internal bar code: | GCACATAGGTTAGCAGGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2405 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATGTCGGTGATGGGTG |
| Suggested primer 2: | AGGGGATGTTGGAATGCTGG |