| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069077 |
| Chromosome: | chromosome 5 |
| Location: | 1038092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247500 | (1 of 2) IPR001841//IPR013083//IPR018957 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type // Zinc finger, C3HC4 RING-type | 3'UTR | |
| Cre05.g247550 | GST7 | (1 of 1) PTHR11260:SF220 - GST-CONTAINING FLYWCH ZINC-FINGER PROTEIN; Glutathione S-transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAAATGCAGCTCGCACGCAATACACTCATTTACGTTGACAACTTCATC |
| Internal bar code: | ATTCGCGTTATCTCAGATAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACATGTCGCAACCTG |
| Suggested primer 2: | TCTGGCACGGTACAAGACAC |