| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069079 |
| Chromosome: | chromosome 7 |
| Location: | 1684208 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325650 | (1 of 3) PF10601 - LITAF-like zinc ribbon domain (zf-LITAF-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGGCGCGCTCCACGTTTAATGGCTGTGCACTGTTGTAAATGGTGCC |
| Internal bar code: | ATATACGCCTAGTTGTTAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTACGGCTGGCACATAC |
| Suggested primer 2: | GCAATGCATAGTTACGCGCA |