Insertion junction: CLIP2.069086_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.069086
Chromosome:chromosome 6
Location:6269680
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292400 SOUL5 (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); DUF2358 domain protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGGAACACGTGGACATGTGGATGCGCCGGAAGCCGCGTACCCAGCGC
Internal bar code:CGCGGGTAATACTAATTCCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2290
LEAP-Seq percent confirming:99.0099
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:101

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGGCTTCAGGTCTTGGTC
Suggested primer 2:TTTGTGTACGGACGCGAAGA