| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069115 |
| Chromosome: | chromosome 6 |
| Location: | 4833733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280200 | (1 of 1) PTHR15141//PTHR15141:SF53 - TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3 // PROTEIN T23E1.3, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACCCAAAACCTCTAACGCCTGCAAAGTTTCGCCTTGGGTCATCACAC |
| Internal bar code: | GTTAACGATACTTATCAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 21.6216 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGCTAGCCCTTCCAGTG |
| Suggested primer 2: | CTATCCGCTGCACCCTAAGG |