Insertion junction: CLIP2.069115_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.069115
Chromosome:chromosome 6
Location:4833737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g280200 (1 of 1) PTHR15141//PTHR15141:SF53 - TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3 // PROTEIN T23E1.3, ISOFORM A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTAAGATGCAGGACGTCATGCTCAGGCACCCAGGTTGACACAGGTAGG
Internal bar code:GTTAACGATACTTATCAGTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1357
LEAP-Seq percent confirming:18.75
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CTATCCGCTGCACCCTAAGG
Suggested primer 2:GTCTGCTAGCCCTTCCAGTG