| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069138 |
| Chromosome: | chromosome 2 |
| Location: | 6816147 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388208 | (1 of 1) 1.13.11.24 - Quercetin 2,3-dioxygenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGAAATATGTTTATGCAAGGTGGGGCGTCCGGGGAGGGCTTTTCTAC |
| Internal bar code: | TAGAAGCCTTGTAGGGATACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1189 |
| LEAP-Seq percent confirming: | 89.0625 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGGAAGAATGCGTCAG |
| Suggested primer 2: | ACCTGTGTGTGTGTGTGTGT |