Insertion junction: CLIP2.069139_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069139
Chromosome:chromosome 8
Location:412964
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g358616 FAP398 Flagellar Associated Protein 398; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCACGCGCTGAACACGTCATCCAGCACACCCGCCACGTCGTTGGGGG
Internal bar code:AACAATGGACGCGCTGCTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:280
LEAP-Seq percent confirming:63.6364
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTCTCCTCCACAACACC
Suggested primer 2:CTGAACCCCAACCCCATACC