| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069140 |
| Chromosome: | chromosome 7 |
| Location: | 5585430 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352000 | (1 of 16) IPR011044 - Quinoprotein amine dehydrogenase, beta chain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTAAATGTCGACAGCAACCAACTACGGTAGTCAACTGCTGGCGATA |
| Internal bar code: | GTTAAAAAGGGACAAGGTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3832 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATCGAGGAGCCAGGTTC |
| Suggested primer 2: | TGTCGTACTTCCAACCCACG |