Insertion junction: CLIP2.069147_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.069147
Chromosome:chromosome 10
Location:2613424
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g436850 (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCACAATGTAGCATTGCCATCGTTCGCCTCGTTAACTGCGTTTCCC
Internal bar code:TCGATTTGTATCAATTAGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:767
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGGTCAGATTGAGGGCA
Suggested primer 2:GAGTTTGCAGCGCACTTCAA