Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.069159 |
Chromosome: | chromosome 3 |
Location: | 8755852 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g207825 | (1 of 1) K03241 - translation initiation factor eIF-2B subunit gamma (EIF2B3) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACCCCAAGCTGTCCAGTCTGCGGCAGGACATGCTGCCCTACCTCACA |
Internal bar code: | GGTTTTGATAGTACCTAAGTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 74 |
LEAP-Seq percent confirming: | 11.1111 |
LEAP-Seq n confirming: | 1 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTACACGCAAACACGGTGTC |
Suggested primer 2: | CCCACATCAAACCCCTCTCC |