| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069161 |
| Chromosome: | chromosome 3 |
| Location: | 623442 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145527 | RBL1 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACGGCTTGCCTTACTCGTGTCGTTGTGTGGCCGTGTGGCCCCGCCCC |
| Internal bar code: | ACAATTTTTGAGCACATTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2251 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCATAAGCGGCAGTCC |
| Suggested primer 2: | CAACGACATGCGCATCTCAG |